Abstract
We describe yeast transcriptional activators encoded by E. coli genomic DNA fragments fused to the coding sequence of the DNA-binding portion of GAL4. All of the new activating sequences that we have analyzed, like those of GAL4 and GCN4, are acidic; most of these sequences show no obvious sequence homology when compared with the identified activating regions of GAL4 and GCN4 or among themselves. We also describe a fusion protein that contains no yeast protein sequence but activates transcription in yeast.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Binding Sites
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DNA / metabolism
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DNA, Bacterial / genetics
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DNA, Recombinant
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DNA-Binding Proteins / genetics*
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Escherichia coli / genetics
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Galactose / metabolism
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Galactose / pharmacology
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Molecular Sequence Data
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Plasmids
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Protein Kinases*
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Recombinant Fusion Proteins / pharmacology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Nucleic Acid
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transcription, Genetic* / drug effects
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beta-Galactosidase / genetics
Substances
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DNA, Bacterial
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DNA, Recombinant
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DNA-Binding Proteins
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Fungal Proteins
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GAL4 protein, S cerevisiae
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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DNA
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Protein Kinases
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beta-Galactosidase
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Galactose