Abstract
Two genes that code for a hapten-specific immunoglobulin M (IgM) have been introduced into the mouse germ line. The transgenic antibody represents 10-50% of the serum IgM and is expressed on the membrane of B cells. B-cell hybridoma lines show that a negative feedback inhibition of mu and kappa transgenic products on the immunoglobulin heavy-chain rearrangement is possible.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Animals
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Base Sequence
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DNA, Recombinant
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Gene Expression Regulation
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Genes
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Hybridomas / immunology
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Immunoglobulin Heavy Chains / genetics*
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Immunoglobulin Light Chains / genetics*
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Immunoglobulin kappa-Chains / genetics*
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Immunoglobulin mu-Chains / genetics*
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Mice
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Plasmids
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RNA, Messenger / genetics
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Recombination, Genetic
Substances
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DNA, Recombinant
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Immunoglobulin Heavy Chains
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Immunoglobulin Light Chains
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Immunoglobulin kappa-Chains
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Immunoglobulin mu-Chains
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RNA, Messenger