Abstract
Rabbit globin mRNA was copied by AMV reverse transcriptase in the presence of various concentrations of deoxyribonucleotides (dNTPs). The cDNAs were analyzed by electrophoresis under denaturing conditions in formamide-polyacrylamide gels. Discrete size products were detected, ranging from 65 to 650 nucleotides-that is, up to the full length of the mRNA template. Increasing the concentrations of all four dNTPs stimulated formation of full-length transcripts and made the incomplete copies less abundant. Hybridization and nuclease digestion experiments indicated that the full-size product is indeed a complete transcript of globin mRNA. Similar results were obtained with chorion mRNAs. The possible usefulness of the discrete partial transcripts is discussed.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Autoradiography
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Avian Leukosis Virus / enzymology
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Centrifugation, Density Gradient
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Chorion
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DNA, Circular / analysis
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DNA, Circular / biosynthesis*
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DNA, Single-Stranded / analysis
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Dactinomycin / pharmacology
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Deoxyribonucleotides / metabolism
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Electrophoresis, Polyacrylamide Gel
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Fibroins
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Globins
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Nucleic Acid Hybridization
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Nucleotides / analysis
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Ovalbumin
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Phosphorus Radioisotopes
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RNA, Messenger / metabolism*
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RNA-Directed DNA Polymerase / metabolism*
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Rabbits
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Templates, Genetic
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Transcription, Genetic*
Substances
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DNA, Circular
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DNA, Single-Stranded
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Deoxyribonucleotides
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Nucleotides
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Phosphorus Radioisotopes
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RNA, Messenger
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Dactinomycin
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Globins
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Ovalbumin
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Fibroins
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RNA-Directed DNA Polymerase