Silver staining of cell bodies by means of physical development

J Neurosci Methods. 1983 Nov;9(3):235-41. doi: 10.1016/0165-0270(83)90086-9.

Abstract

A method for silver impregnation of cell bodies is detailed. It is based on Gallyas' physical development procedure, and adds a silver stain for cell bodies to the list of histological elements stainable with high contrast by means of physical development. Its black pigment on a clear background aids visibility, localization of cytoarchitectonic boundaries, and photographic reproduction. It also stains regions of tissue damage, such as tracks from micropipettes or electrodes. A manipulation for enhancement of dendritic staining is also described.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain / anatomy & histology*
  • Cerebral Cortex / anatomy & histology
  • Cricetinae
  • Dendrites / ultrastructure
  • Hippocampus / anatomy & histology
  • Histological Techniques*
  • Male
  • Mesocricetus
  • Neurons / ultrastructure
  • Silver Nitrate*
  • Staining and Labeling / methods*
  • Superior Colliculi / anatomy & histology

Substances

  • Silver Nitrate