The present paper has reviewed several factors related to ion transport and examined the properties of cation transport in mitochondria. The analysis suggests that: (1) The concept that a metabolically dependent electrical potential across the mitochondrial membrane plays a role in determining ion fluxes and steady-state concentrations is not justified and the data indicate that such exchanges are generally electroneutral. (2) Generally, the influx and efflux of an ion proceed by the same mechanism with at least one exception. (3) There are indications that some of the steps in transport are common to several cations. (4) The idea that carrier or ionophoric molecules are involved in cation transport has been examined in some detail together with the possible involvement of some known mitochondrial components. In particular, a model has been introduced in which local charge imbalances produced by H+ fluxes serve as the driving force of transport. The molecules of the complex are arranged in series in a tripartite arrangement including a filter or gate, a nonselective channel and an H+-transferring portion linked to either electron transport or the ATPase. Parts of this model have been introduced by other investigators. Models in which different portions of channels have differing functions have been proposed previously for other transport systems.