Alteration of the circulating life and antigenic properties of bovine adenosine deaminase in mice by attachment of polyethylene glycol

Clin Exp Immunol. 1981 Dec;46(3):649-52.

Abstract

Polyethylene glycol was attached covalently to adenosine deaminase (ADA) using cyanuric chloride as the coupling agent. The modified adenosine deaminase (PEG-ADA) appears to lose its immunogenicity in mice following multiple intravenous injections. PEG-ADA does not react with antibodies raised against native ADA. The circulating half-life (T1/2) of PEG-ADA was increased to 28 hr. The lack of detectable antibody formation and long circulating life may make PEG-ADA suitable for treating human ADA deficiency.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Deaminase / blood
  • Adenosine Deaminase / immunology*
  • Animals
  • Antibody Formation
  • Antigens*
  • Cattle
  • Cross-Linking Reagents
  • Half-Life
  • Mice
  • Nucleoside Deaminases / immunology*
  • Polyethylene Glycols / blood
  • Polyethylene Glycols / immunology*
  • Triazines

Substances

  • Antigens
  • Cross-Linking Reagents
  • Triazines
  • Polyethylene Glycols
  • cyanuric chloride
  • Nucleoside Deaminases
  • Adenosine Deaminase