Abstract
The notochord is a midline mesodermal structure with an essential patterning function in all vertebrate embryos. Zebrafish floating head (flh) mutants lack a notochord, but develop with prechordal plate and other mesodermal derivatives, indicating that flh functions specifically in notochord development. We show that floating head is the zebrafish homologue of Xnot, a homeobox gene expressed in the amphibian organizer and notochord. We propose that flh regulates notochord precursor cell fate.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Differentiation / genetics
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Chromosome Mapping
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DNA Primers
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DNA-Binding Proteins / genetics
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Gastrula / cytology
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Genes, Homeobox*
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Genes, Lethal
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Hedgehog Proteins
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Homeodomain Proteins / genetics*
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Humans
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Mesoderm / cytology
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Molecular Sequence Data
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Motor Neurons / cytology
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Mutation
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Notochord / embryology*
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Proteins / genetics
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RNA, Messenger / biosynthesis
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Stem Cells / cytology
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Tail
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Trans-Activators / genetics
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Transcription Factors / genetics*
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Xenopus Proteins*
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Zebrafish
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Zebrafish Proteins*
Substances
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DNA Primers
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DNA-Binding Proteins
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Hedgehog Proteins
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Homeodomain Proteins
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Not protein, Xenopus
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Proteins
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RNA, Messenger
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Trans-Activators
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Transcription Factors
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Xenopus Proteins
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Zebrafish Proteins
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noto protein, zebrafish