Abstract
Chromatin assembly factor I (CAF-I) from human cell nuclei is a three-subunit protein complex that assembles histone octamers onto replicating DNA in a cell-free system. Sequences of cDNAs encoding the two largest CAF-I subunits reveal that the p150 protein contains large clusters of charged residues, whereas p60 contains WD repeats. p150 and p60 directly interact and are both required for DNA replication-dependent assembly of nucleosomes. Deletion of the p60-binding domain from the p150 protein prevents chromatin assembly. p150 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in human cell extracts, suggesting that such complexes are intermediates between histone synthesis and assembly onto replicating DNA.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Line
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Cell Nucleus / metabolism*
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Chromatin Assembly Factor-1
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Chromosomal Proteins, Non-Histone*
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Cloning, Molecular
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DNA Replication*
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / isolation & purification
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DNA-Binding Proteins / metabolism*
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Histones / biosynthesis
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Histones / isolation & purification
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Histones / metabolism*
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Humans
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Macromolecular Substances
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Protein Binding
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Protein Biosynthesis
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Repetitive Sequences, Nucleic Acid
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Sequence Deletion
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Transcription Factors
Substances
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CHAF1B protein, human
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CNOT8 protein, human
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Chromatin Assembly Factor-1
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Histones
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Macromolecular Substances
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Oligodeoxyribonucleotides
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Recombinant Proteins
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Transcription Factors
Associated data
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GENBANK/U20979
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GENBANK/U20980