Abstract
A gene encoding a novel cdc2-related protein kinase has been identified in Plasmodium falciparum, using degenerate oligonucleotides designed to hybridise to regions that are conserved in members of the cdc2 gene family. This gene, called Pfcrk-1, is located on chromosome 4. It is most closely related to the p58GTA gene family, members of which are negative regulators of cell growth in vertebrates. Pfcrk-1 is developmentally regulated, as indicated by stage-specific accumulation of mRNA in gametocytes.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
CDC2 Protein Kinase / biosynthesis
-
CDC2 Protein Kinase / genetics*
-
CDC2 Protein Kinase / isolation & purification
-
Cell Cycle
-
Chromosome Mapping
-
Electrophoresis, Gel, Pulsed-Field
-
Enzyme Induction
-
Female
-
Gene Expression Regulation, Developmental
-
Male
-
Molecular Sequence Data
-
Multigene Family
-
Plasmodium falciparum / enzymology*
-
Plasmodium falciparum / genetics
-
Plasmodium falciparum / growth & development
-
Protozoan Proteins / biosynthesis
-
Protozoan Proteins / genetics*
-
Protozoan Proteins / isolation & purification
-
RNA, Messenger / biosynthesis
-
RNA, Messenger / genetics
-
RNA, Protozoan / biosynthesis
-
RNA, Protozoan / genetics
-
Sequence Alignment
-
Sequence Homology
-
Sequence Homology, Amino Acid
Substances
-
Protozoan Proteins
-
RNA, Messenger
-
RNA, Protozoan
-
CDC2 Protein Kinase
-
Pfcrk-1 protein, Plasmodium falciparum