pBINPLUS: an improved plant transformation vector based on pBIN19

F A van Engelen; J W Molthoff; A J Conner; J P Nap; A Pereira; W J Stiekema

doi:10.1007/BF01969123

pBINPLUS: an improved plant transformation vector based on pBIN19

Transgenic Res. 1995 Jul;4(4):288-90. doi: 10.1007/BF01969123.

Authors

F A van Engelen¹, J W Molthoff, A J Conner, J P Nap, A Pereira, W J Stiekema

Affiliation

¹ Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Molecular Biology, Wageningen, The Netherlands.

PMID: 7655517
DOI: 10.1007/BF01969123

Abstract

We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium tumefaciens / genetics*
Amino Acid Oxidoreductases / genetics
Base Sequence
Cloning, Molecular / methods
DNA, Bacterial / genetics*
Genetic Vectors*
Kanamycin Kinase
Molecular Sequence Data
Phosphotransferases (Alcohol Group Acceptor) / genetics
Plants / genetics*
Plants, Genetically Modified
Plasmids
Replication Origin
Transformation, Genetic*

Substances

DNA, Bacterial
Amino Acid Oxidoreductases
nopaline synthase
Phosphotransferases (Alcohol Group Acceptor)
Kanamycin Kinase