Abstract
Ribonucleotide reductase is the only enzyme that catalyses de novo formation of deoxyribonucleotides and is thus a key enzyme in DNA synthesis. The radical-based reaction involves five cysteins. Two redox-active cysteines are located at adjacent antiparallel strands in a new type of ten-stranded alpha/beta-barrel, and two others at the carboxyl end in a flexible arm. The fifth cysteine, in a loop in the centre of the barrel, is positioned to initiate the radical reaction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Binding Sites
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Crystallography, X-Ray
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Cysteine
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Escherichia coli / enzymology
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Models, Chemical
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Models, Molecular
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Molecular Sequence Data
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Peptide Fragments / metabolism
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Protein Conformation
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Protein Structure, Secondary
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Ribonucleotide Reductases / chemistry*
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Ribonucleotide Reductases / metabolism
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Sequence Homology, Amino Acid
Substances
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Peptide Fragments
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Ribonucleotide Reductases
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Cysteine