Abstract
Genetic control elements are usually situated in local regions of chromatin that are hypersensitive to structural probes such as DNase I. We have reconstructed the chromatin structure of the hsp70 promoter using an in vitro nucleosome assembly system. Binding of the GAGA transcription factor on existing nucleosomes leads to nucleosome disruption, DNase I hypersensitivity at the TATA box and heat-shock elements, and rearrangement of adjacent nucleosomes. ATP hydrolysis facilitates this process, suggesting that an energy-dependent pathway is involved in chromatin remodelling.
MeSH terms
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Adenosine Triphosphate / metabolism*
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Animals
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Chromatin / metabolism*
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Chromosomes / metabolism
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DNA-Binding Proteins / metabolism*
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Deoxyribonuclease I / metabolism
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Drosophila
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Drosophila Proteins*
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Heat-Shock Proteins / genetics*
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Histones / metabolism
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Homeodomain Proteins*
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Larva
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Nucleosomes / metabolism*
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Promoter Regions, Genetic*
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Protein Binding
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Recombinant Proteins / metabolism
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Transcription Factors / metabolism*
Substances
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Chromatin
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DNA-Binding Proteins
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Drosophila Proteins
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Heat-Shock Proteins
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Histones
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Homeodomain Proteins
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Nucleosomes
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Recombinant Proteins
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Transcription Factors
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Trl protein, Drosophila
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Adenosine Triphosphate
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Deoxyribonuclease I