Abstract
Transcription of a putative mitochondrial gene (orf138) has previously been correlated with Ogura cytoplasmic male-sterility (CMS) in rapeseed cybrids. In this paper, studies performed on a Brassica cybrid with a different organization of the orf138 locus confirm this association. We also show that mitochondria isolated from male-sterile rapeseed plants synthesize a polypeptide of 19 kDa, which is absent in fertile revertants. Antibodies against a glutathione S-transferase-ORF138 fusion protein were raised to establish that this 19 kDa polypeptide is the product of orf138. The anti-ORF138 serum was used to demonstrate that the orf138 translation product occurs only in sterile cybrids and co-purifies with the mitochondrial membrane fraction.
MeSH terms
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Blotting, Western
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Brassica / genetics*
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Brassica / physiology
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Cloning, Molecular
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DNA, Mitochondrial / biosynthesis*
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Deoxyribonuclease BamHI
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Deoxyribonucleases, Type II Site-Specific
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Electrophoresis, Polyacrylamide Gel
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Fertility / genetics
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Hybridization, Genetic
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Intracellular Membranes / chemistry
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Membrane Proteins / biosynthesis*
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Membrane Proteins / genetics
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Molecular Sequence Data
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Open Reading Frames
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Plant Proteins / biosynthesis*
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Plant Proteins / genetics
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Plants / genetics*
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Precipitin Tests
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Protein Biosynthesis
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Recombinant Fusion Proteins / biosynthesis
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Restriction Mapping
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Transcription, Genetic
Substances
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DNA, Mitochondrial
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Membrane Proteins
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Plant Proteins
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Recombinant Fusion Proteins
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Deoxyribonuclease BamHI
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endodeoxyribonuclease NcoI
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Deoxyribonucleases, Type II Site-Specific