This article reviews the use of fluorescent probes to monitor the order and dynamics within the acyl chain region of liposome lipid bilayers. Fluorescence anisotropy is first defined and the theoretical framework that allows interpretation of steady-state or dynamic measurements in terms of molecular details is reviewed. The general advantages and/or limitations of fluorescent versus other methods of monitoring membrane order and dynamics are discussed. The properties of two classes of fluorescence probes are then described. The linear probes 1,6-diphenyl-1,3,5-hexatriene (DPH) and parinaric acid (PA) and their derivatives are seen as particularly useful when quantitative interpretation of observations in terms of details of bilayer dynamics and order are critical. Of these, DPH is the more widely and easily used, although parinaric acid has advantages for certain applications. The non-linear probes considered include the anthroyloxyl fatty acids and the recently introduced fluorenyl fatty acid probes. While the geometry and electronic configurations of these probes do not allow for detailed molecular interpretations, these probes can provide unique qualitative information about the state of the lipid bilayer at various positions along the acyl chains.