Abstract
We ascertained by the stopped flow method the overall association rate constant, k+1, of filamin and alpha-actinin to fluorescently labelled filamentous actin of approximately 1.3 x 10(6) M-1.s-1 and approximately 1.0 x 10(6) M-1.s-1 as well as the overall dissociation rate constant, k-1, of approximately 0.6 s-1 and approximately 0.4 s-1, respectively. The overall equilibrium constant, K, for filamin and alpha-actinin to actin deduced from the relation K = k+1/k-1 agree well with published data.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinin / chemistry*
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Actinin / metabolism
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Actins / chemistry*
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Actins / metabolism
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Animals
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Binding, Competitive
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Carrier Proteins / chemistry
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Chickens
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Contractile Proteins / chemistry*
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Contractile Proteins / metabolism
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Filamins
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Gizzard, Avian
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Kinetics
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Microfilament Proteins / chemistry*
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Microfilament Proteins / metabolism
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Muscle, Smooth / metabolism
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Muscles / metabolism
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Rabbits
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Turkeys
Substances
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Actins
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Carrier Proteins
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Contractile Proteins
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Filamins
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Microfilament Proteins
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Actinin