Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyl transferase activity

EMBO J. 1993 Jan;12(1):279-84. doi: 10.1002/j.1460-2075.1993.tb05654.x.

Abstract

Asparagine-linked N-glycosylation is an essential protein modification occurring in all eukaryotic cells. The central step is the co-translational transfer of the core oligosaccharide assembled on the lipid carrier dolichol phosphate to selected Asn-X-Ser/Thr residues of nascent polypeptide chains in the endoplasmic reticulum. This reaction is catalyzed by the enzyme N-oligosaccharyl transferase. In yeast, Wbp1p is an essential component of this enzyme. Using a high copy number suppression approach, the SWP1 gene was isolated as an allele specific suppressor of a wbp1 mutation. Swp1p is a 30 kDa type I transmembrane protein and essential for cell viability. Similar to Wbp1p, depletion of Swp1p results in reduced N-oligosaccharyl transferase activity in vivo and in vitro. Wbp1p and Swp1p can be chemically cross-linked, suggesting that both proteins are essential constituents of the N-oligosaccharyl transferase complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Fungal Proteins / genetics*
  • Fungal Proteins / isolation & purification
  • Fungal Proteins / metabolism
  • Genes, Fungal*
  • Hexosyltransferases*
  • Kinetics
  • Macromolecular Substances
  • Membrane Proteins / genetics*
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Molecular Weight
  • Oligodeoxyribonucleotides
  • Phenotype
  • Plasmids
  • Protein Biosynthesis
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics*
  • Suppression, Genetic
  • Temperature
  • Transferases / genetics*
  • Transferases / isolation & purification
  • Transferases / metabolism*

Substances

  • Fungal Proteins
  • Macromolecular Substances
  • Membrane Proteins
  • Oligodeoxyribonucleotides
  • Recombinant Proteins
  • Transferases
  • Hexosyltransferases
  • dolichyl-diphosphooligosaccharide - protein glycotransferase

Associated data

  • GENBANK/L09676
  • GENBANK/L09677
  • GENBANK/L09678
  • GENBANK/L09679
  • GENBANK/L09680
  • GENBANK/L09681
  • GENBANK/L09682
  • GENBANK/L09683
  • GENBANK/S57804
  • GENBANK/X67705