Abstract
Asparagine-linked N-glycosylation is an essential protein modification occurring in all eukaryotic cells. The central step is the co-translational transfer of the core oligosaccharide assembled on the lipid carrier dolichol phosphate to selected Asn-X-Ser/Thr residues of nascent polypeptide chains in the endoplasmic reticulum. This reaction is catalyzed by the enzyme N-oligosaccharyl transferase. In yeast, Wbp1p is an essential component of this enzyme. Using a high copy number suppression approach, the SWP1 gene was isolated as an allele specific suppressor of a wbp1 mutation. Swp1p is a 30 kDa type I transmembrane protein and essential for cell viability. Similar to Wbp1p, depletion of Swp1p results in reduced N-oligosaccharyl transferase activity in vivo and in vitro. Wbp1p and Swp1p can be chemically cross-linked, suggesting that both proteins are essential constituents of the N-oligosaccharyl transferase complex.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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Escherichia coli / genetics
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Fungal Proteins / genetics*
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Fungal Proteins / isolation & purification
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Fungal Proteins / metabolism
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Genes, Fungal*
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Hexosyltransferases*
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Kinetics
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Macromolecular Substances
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Membrane Proteins / genetics*
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Membrane Proteins / isolation & purification
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Molecular Weight
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Oligodeoxyribonucleotides
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Phenotype
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Plasmids
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Protein Biosynthesis
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Restriction Mapping
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics*
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Suppression, Genetic
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Temperature
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Transferases / genetics*
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Transferases / isolation & purification
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Transferases / metabolism*
Substances
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Fungal Proteins
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Macromolecular Substances
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Membrane Proteins
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Oligodeoxyribonucleotides
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Recombinant Proteins
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Transferases
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Hexosyltransferases
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dolichyl-diphosphooligosaccharide - protein glycotransferase
Associated data
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GENBANK/L09676
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GENBANK/L09677
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GENBANK/L09678
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GENBANK/L09679
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GENBANK/L09680
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GENBANK/L09681
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GENBANK/L09682
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GENBANK/L09683
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GENBANK/S57804
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GENBANK/X67705