Abstract
During meiosis, branched DNA molecules containing information from both parental chromosomes occur in vivo at loci where meiosis-specific double-stranded breaks occur. We demonstrate here that these joint molecules are recombination intermediates: they contain single strands that have undergone exchange of information. Moreover, these joint molecules are resolved into both parental and recombinant duplexes when treated in vitro with Holliday junction-resolving endonucleases RuvC or T4 endo VII. Taken together with previous observations, these results strongly suggest that joint molecules are double Holliday junctions.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alcohol Oxidoreductases
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Aminohydrolases
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Bacterial Proteins
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Cross-Linking Reagents
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Crossing Over, Genetic / genetics
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DNA, Fungal / chemistry
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DNA, Fungal / genetics
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DNA, Single-Stranded / chemistry
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DNA, Single-Stranded / genetics*
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Endodeoxyribonucleases
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Escherichia coli Proteins*
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Ficusin
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Fungal Proteins / genetics
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Meiosis / genetics*
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Models, Genetic
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Nucleic Acid Conformation
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Nucleic Acid Heteroduplexes / genetics
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Pyrophosphatases
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Recombination, Genetic / genetics*
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Saccharomyces cerevisiae Proteins*
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Transcription Factors / genetics
Substances
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Bacterial Proteins
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Cross-Linking Reagents
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DNA, Fungal
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DNA, Single-Stranded
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Escherichia coli Proteins
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Fungal Proteins
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Nucleic Acid Heteroduplexes
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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ruvC protein, E coli
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Alcohol Oxidoreductases
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HIS4 protein, S cerevisiae
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Endodeoxyribonucleases
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endodeoxyribonuclease VII
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Aminohydrolases
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Pyrophosphatases
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Ficusin