Requirement for Xist in X chromosome inactivation

G D Penny; G F Kay; S A Sheardown; S Rastan; N Brockdorff

doi:10.1038/379131a0

Requirement for Xist in X chromosome inactivation

Nature. 1996 Jan 11;379(6561):131-7. doi: 10.1038/379131a0.

Authors

G D Penny¹, G F Kay, S A Sheardown, S Rastan, N Brockdorff

Affiliation

¹ Section of Comparative Biology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.

PMID: 8538762
DOI: 10.1038/379131a0

Abstract

The Xist gene has been proposed as a candidate for the X inactivation centre, the master regulatory switch locus that controls X chromosome inactivation. So far this hypothesis has been supported solely by indirect evidence. Here we describe gene targeting of Xist, and provide evidence for its absolute requirement in the process of X chromosome inactivation.

MeSH terms

Alleles
Animals
Base Sequence
Cell Line
Chimera
Clone Cells
DNA Primers
Dosage Compensation, Genetic*
Embryo, Mammalian
Female
Gene Targeting
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Molecular Sequence Data
Mutagenesis
RNA, Long Noncoding
RNA, Untranslated*
Restriction Mapping
Stem Cells
Transcription Factors / genetics*
X Chromosome / genetics

Substances

DNA Primers
RNA, Long Noncoding
RNA, Untranslated
Transcription Factors
XIST non-coding RNA