Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae

J O Mascorro-Gallardo; A A Covarrubias; R Gaxiola

doi:10.1016/0378-1119(96)00059-5

Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae

Gene. 1996 Jun 12;172(1):169-70. doi: 10.1016/0378-1119(96)00059-5.

Authors

J O Mascorro-Gallardo¹, A A Covarrubias, R Gaxiola

Affiliation

¹ Departamento de Biología Molecular de Plantas, UNAM, Cuernavaca, Morelos, Mexico.

PMID: 8654982
DOI: 10.1016/0378-1119(96)00059-5

Abstract

We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Carrier Proteins
Cloning, Molecular
DNA, Fungal
Gene Expression Regulation, Fungal*
Genetic Vectors*
Metallothionein / genetics*
Molecular Sequence Data
Promoter Regions, Genetic*
Saccharomyces cerevisiae / genetics*

Substances

CUP1-1 protein, S cerevisiae
Carrier Proteins
DNA, Fungal
copper thionein
Metallothionein