Abstract
Chromatin assembly factor 1 (CAF-1) assembles nucleosomes in a replication-dependent manner. The small subunit of CAF-1 (p48) is a member of a highly conserved subfamily of WD-repeat proteins. There are at least two members of this subfamily in both human (p46 and p48) and yeast cells (Hat2p, a subunit of the B-type H4 acetyltransferase, and Msi1p). Human p48 can bind to histone H4 in the absence of CAF-1 p150 and p60. p48, also a known subunit of a histone deacetylase, copurifies with a chromatin assembly complex (CAC), which contains the three subunits of CAF-1 (p150, p60, p48) and H3 and H4, and promotes DNA replication-dependent chromatin assembly. CAC histone H4 exhibits a novel pattern of lysine acetylation that overlaps with, but is distinct from, that reported for newly synthesized H4 isolated from nascent chromatin. Our data suggest that CAC is a key intermediate of the de novo nucleosome assembly pathway and that the p48 subunit participates in other aspects of histone metabolism.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylation
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Acetyltransferases / metabolism
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Amino Acid Sequence
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Cell Extracts
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Cell Line
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Cell Nucleus
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Chromatin / metabolism
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Chromatin Assembly Factor-1
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Chromosomal Proteins, Non-Histone*
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Cloning, Molecular
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Cytoplasm
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DNA Replication / physiology
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DNA, Complementary / genetics
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / isolation & purification
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DNA-Binding Proteins / metabolism*
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Histone Acetyltransferases
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Histones / chemistry
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Histones / isolation & purification
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Histones / metabolism*
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Humans
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Lysine / metabolism
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Molecular Sequence Data
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Molecular Weight
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Nucleosomes / metabolism*
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Saccharomyces cerevisiae Proteins*
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Transcription Factors
Substances
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CHAF1B protein, human
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CNOT8 protein, human
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Cell Extracts
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Chromatin
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Chromatin Assembly Factor-1
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Chromosomal Proteins, Non-Histone
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DNA, Complementary
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DNA-Binding Proteins
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Histones
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Nucleosomes
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Acetyltransferases
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Histone Acetyltransferases
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Lysine