Abstract
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Abscisic Acid / biosynthesis
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Abscisic Acid / genetics*
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Arabidopsis / drug effects
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Arabidopsis / genetics*
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Arabidopsis / radiation effects
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Chromosome Mapping
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Crosses, Genetic
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Darkness
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Dose-Response Relationship, Drug
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Drug Resistance
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Genes, Plant*
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Genes, Recessive
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Germination
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Light
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Meiosis
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Mutation*
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Polyethylene Glycols / pharmacology
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Selection, Genetic
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Sodium Chloride / pharmacology
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Triazoles / pharmacology
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Water / metabolism
Substances
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Triazoles
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Water
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Polyethylene Glycols
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Sodium Chloride
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paclobutrazol
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Abscisic Acid