Enhanced DNA extraction and PCR amplification of mitochondrial genes from formalin-fixed museum specimens

Biotechniques. 1997 Mar;22(3):394-6, 398, 400. doi: 10.2144/97223bm03.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohols
  • Animals
  • Base Sequence
  • Cell Extracts / chemistry
  • Classification
  • Cloning, Molecular
  • DNA Damage
  • DNA Primers
  • DNA, Mitochondrial / genetics
  • DNA, Mitochondrial / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel
  • Endopeptidases / metabolism
  • Formaldehyde
  • Mitochondria / genetics*
  • Molecular Sequence Data
  • Museums
  • Paleontology / methods
  • Polymerase Chain Reaction*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis
  • Sequence Homology, Nucleic Acid
  • Tissue Fixation

Substances

  • Alcohols
  • Cell Extracts
  • DNA Primers
  • DNA, Mitochondrial
  • RNA, Ribosomal, 16S
  • Formaldehyde
  • Endopeptidases