Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins

Cell. 1997 Jun 27;89(7):1133-44. doi: 10.1016/s0092-8674(00)80300-1.

Abstract

Mutations in the Arabidopsis ETHYLENE-INSENSITIVE3 (EIN3) gene severely limit a plant's response to the gaseous hormone ethylene. ein3 mutants show a loss of ethylene-mediated effects including gene expression, the triple response, cell growth inhibition, and accelerated senescence. EIN3 acts downstream of the histidine kinase ethylene receptor, ETR1, and the Raf-like kinase, CTR1. The EIN3 gene encodes a novel nuclear-localized protein that shares sequence similarity, structural features, and genetic function with three EIN3-LIKE (EIL) proteins. In addition to EIN3, EIL1 orEIL2 were able to complement ein3, suggesting their participation in the ethylene signaling pathway. Overexpression of EIN3 or EIL1 in wild-type or ethylene-insensitive2 plants conferred constitutive ethylene phenotypes, indicating their sufficiency for activation of the pathway in the absence of ethylene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Arabidopsis / genetics
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins*
  • Base Sequence
  • Cell Nucleus / chemistry
  • Cloning, Molecular
  • Conserved Sequence*
  • DNA-Binding Proteins
  • Ethylenes / metabolism*
  • Gene Expression Regulation, Developmental / physiology
  • Gene Expression Regulation, Plant / physiology
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Mutagenesis / physiology
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Nuclear Proteins / physiology*
  • Phenotype
  • Plant Development
  • Plant Growth Regulators / metabolism
  • Plants / genetics
  • Plants / metabolism
  • Sequence Homology, Amino Acid
  • Transcription Factors*

Substances

  • Arabidopsis Proteins
  • DNA-Binding Proteins
  • EIN3 protein, Arabidopsis
  • Ethylenes
  • Nuclear Proteins
  • Plant Growth Regulators
  • Transcription Factors
  • ethylene

Associated data

  • GENBANK/AF004213
  • GENBANK/AF004214
  • GENBANK/AF004215
  • GENBANK/AF004216
  • GENBANK/AF004217