Abstract
Platelet activation occurs in early pregnancy in women at risk of developing pre-eclampsia. Cytokines have been implicated in the pathogenesis of pre-eclampsia, so we determined the effects of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) on the in vitro aggregation of human platelets. IL-1beta increased aggregation of platelets from non-pregnant and pre-eclamptic women, and inhibited the aggregation of platelets from normal pregnant women. This latter effect was linked to a diminished P-selectin expression on ADP-stimulated whole blood platelets in normal pregnant women (p = 0.011). Platelet aggregation in response to ADP was found to be inhibited after preincubation with TNF-alpha in non-pregnant (38%, p = 0.01) and in normal pregnant women (54%, p = 0.001) and not affected in pre-eclamptic women. The inhibitory effects of TNF-alpha were mediated through the P75 receptor for TNF-alpha.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenosine Diphosphate / pharmacology
-
Adult
-
Blood Platelets / drug effects
-
Blood Platelets / metabolism
-
Cyclic AMP / biosynthesis
-
Cyclic GMP / biosynthesis
-
Female
-
Humans
-
Interleukin 1 Receptor Antagonist Protein
-
Interleukin-1 / pharmacology*
-
Middle Aged
-
Nitric Oxide / biosynthesis
-
P-Selectin / blood
-
Platelet Aggregation / drug effects*
-
Pre-Eclampsia / blood*
-
Pregnancy / blood*
-
Pregnancy Complications, Cardiovascular / blood*
-
Recombinant Proteins / pharmacology
-
Second Messenger Systems / drug effects
-
Sialoglycoproteins / pharmacology
-
Thromboxane B2 / biosynthesis
-
Tumor Necrosis Factor-alpha / pharmacology*
Substances
-
IL1RN protein, human
-
Interleukin 1 Receptor Antagonist Protein
-
Interleukin-1
-
P-Selectin
-
Recombinant Proteins
-
Sialoglycoproteins
-
Tumor Necrosis Factor-alpha
-
Nitric Oxide
-
Thromboxane B2
-
Adenosine Diphosphate
-
Cyclic AMP
-
Cyclic GMP