Combination of a viability test and a quantification method for Schistosoma haematobium eggs (filtration--trypan blue-staining-technique)

Tropenmed Parasitol. 1979 Dec;30(4):417-22.

Abstract

The efficiency of the polycarbamate membrane filtration technique for detecting Schistosoma haematobium eggs in urine has been increased by using a pore size of 14 micrometers diameter and the suction of a water jet pump. The technique permits filtration of several 1000 ml of urine in one test. Eggs concentrations of 1 egg in more than 1000 ml of urine were detected. Viability was assess after filtration by staining with trypan blue. Preliminary data of 118 Schistosoma haematobium-positive urine samples of 32 patients suggest that the filtration trypan blue-staining-technique is of high validity in light infection with scanty egg-output.

MeSH terms

  • Filtration / methods
  • Humans
  • Larva / growth & development
  • Parasite Egg Count / methods*
  • Schistosoma haematobium / growth & development
  • Schistosomiasis / diagnosis*
  • Schistosomiasis / drug therapy
  • Schistosomiasis / urine
  • Staining and Labeling
  • Trichlorfon / therapeutic use

Substances

  • Trichlorfon