Abstract
The Na+-H+ exchanger regulatory factor (NHE-RF) is a cytoplasmic phosphoprotein that was first found to be involved in protein kinase A mediated regulation of ion transport. NHE-RF contains two distinct protein interaction PDZ domains: NHE-RF-PDZ1 and NHE-RF-PDZ2. However, their binding partners are currently unknown. Because PDZ domains usually bind to specific short linear C-terminal sequences, we have carried out affinity selection of random peptides for specific sequences that interact with the NHE-RF PDZ domains and found that NHE-RF-PDZ1 is capable of binding to the CFTR C-terminus. The specific and tight association suggests a potential regulatory role of NHE-RF in cystic fibrosis transmembrane conductance regulator (CFTR) function.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Consensus Sequence
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Cystic Fibrosis Transmembrane Conductance Regulator / chemistry
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Cystic Fibrosis Transmembrane Conductance Regulator / genetics*
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Cystic Fibrosis Transmembrane Conductance Regulator / metabolism
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Drosophila Proteins*
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Insect Proteins / chemistry
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Mice
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Molecular Sequence Data
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Phosphoproteins / chemistry
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Phosphoproteins / genetics*
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Phosphoproteins / metabolism
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Protein Conformation
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Sequence Alignment
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Sequence Homology, Amino Acid
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Sodium-Hydrogen Exchangers
Substances
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Drosophila Proteins
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Insect Proteins
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Phosphoproteins
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Sodium-Hydrogen Exchangers
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cno protein, Drosophila
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sodium-hydrogen exchanger regulatory factor
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Cystic Fibrosis Transmembrane Conductance Regulator