Abstract
A paralog (intraspecies homolog) of the Saccharomyces cerevisiae YEF3 gene, encoding elongation factor-3, has been sequenced in the course of the yeast genome project, and identified by database searching; this gene has been designated HEF3. Bioinformatic and Northern blot analysis indicate that the HEF3 gene is not expressed during vegetative growth. Deletion of the HEF3 gene reveals no growth defects, nor any defects in mating or sporulation. A high copy 2 mu clone of HEF3 was constructed, and was shown to be unable to complement a null allele of yef3. Finally, an in vitro assay for ribosome-stimulated ATPase activity was performed with isogenic HEF3 and delta hef3 strains; no difference in biochemical activity could be detected in these strains. From these results, we conclude that the HEF3 gene does not encode a functional homolog of YEF3.
MeSH terms
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Adenosine Triphosphatases / metabolism
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Amino Acid Sequence
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Blotting, Northern
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Cloning, Molecular
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Computational Biology
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Conserved Sequence / genetics*
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Databases, Factual
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Fungal Proteins*
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Gene Expression
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Genes, Essential / genetics
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Genes, Fungal / genetics*
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Genetic Complementation Test
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Genetic Vectors / genetics
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Peptide Elongation Factors / genetics*
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Phenotype
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Plasmids / genetics
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Recombinant Fusion Proteins / genetics
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Ribosomes / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Deletion
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Sequence Homology, Amino Acid
Substances
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Fungal Proteins
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Peptide Elongation Factors
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RNA, Messenger
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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YEF3 protein, S cerevisiae
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Adenosine Triphosphatases