[Preparation and analysis of monoclonal antibody against EphA4 peptide]

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Oct;30(5):529-32.
[Article in Chinese]

Abstract

Objective: To prepare monoclonal antibody (MAb) against carboxy-terminus of E-phA4 and to analyze the immunological characteristics and significance of the antibody.

Methods: Mice were immunized with a chemically synthesized peptide that had been conjugated to KLH (keyhole limpet hemocyamin). A panel of antibodies specifically against EphA4 were obtained by hybridoma technique. The immunological properties and significance of the MAb were analyzed by immunological and immunochemistry techniques.

Results: A hybridoma cell line secreting anti-EphA4 MAb was established. The MAb reacted specifically with human, chicken and mouse EphA4, but did not cross-react with EphA5 and EphA7. The antibody could be used for immunochemical techniques such as ELISA, immunoprecipitation, Western blot and immunohistochemistry.

Conclusion: The anti-EphA4 MAb generated by immunizing a synthetic peptide possesses excellent immunological properties. MAb can be applied for immunological and immunohistochemical purpose and will become an important tool in the study of EphA4 and its ligand.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Hybridomas
  • Mice
  • Mice, Inbred BALB C
  • Peptide Fragments / immunology
  • Receptor, EphA4 / immunology*

Substances

  • Antibodies, Monoclonal
  • Peptide Fragments
  • Receptor, EphA4