A Rabbit Monoclonal Antibody against the Antiviral and Cancer Genomic DNA Mutating Enzyme APOBEC3B

William L Brown; Emily K Law; Prokopios P Argyris; Michael A Carpenter; Rena Levin-Klein; Alison N Ranum; Amy M Molan; Colleen L Forster; Brett D Anderson; Lela Lackey; Reuben S Harris

doi:10.3390/antib8030047

A Rabbit Monoclonal Antibody against the Antiviral and Cancer Genomic DNA Mutating Enzyme APOBEC3B

Antibodies (Basel). 2019 Sep 10;8(3):47. doi: 10.3390/antib8030047.

Authors

William L Brown^{1

2

3

4}, Emily K Law^{1

2

3

4

5}, Prokopios P Argyris^{1

2

3

4

6}, Michael A Carpenter^{1

2

3

4

5}, Rena Levin-Klein^{1

2

3

4}, Alison N Ranum^{1

2

3

4}, Amy M Molan^{1

2

3

4}, Colleen L Forster⁷, Brett D Anderson^{1

2

3

4}, Lela Lackey^{1

2

3

4

8}, Reuben S Harris^{9

10

11

12

13}

Affiliations

¹ Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
² Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
³ Institute for Molecular Virology, University of Minnesota, Minneapolis, MN 55455, USA.
⁴ Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA.
⁵ Howard Hughes Medical Institute, University of Minnesota, Minneapolis, MN 55455, USA.
⁶ Division of Oral and Maxillofacial Pathology, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA.
⁷ Clinical and Translational Science Institute, University of Minnesota, Minneapolis, MN 55455, USA.
⁸ Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA.
⁹ Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA. rsh@umn.edu.
¹⁰ Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA. rsh@umn.edu.
¹¹ Institute for Molecular Virology, University of Minnesota, Minneapolis, MN 55455, USA. rsh@umn.edu.
¹² Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA. rsh@umn.edu.
¹³ Howard Hughes Medical Institute, University of Minnesota, Minneapolis, MN 55455, USA. rsh@umn.edu.

Abstract

The DNA cytosine deaminase APOBEC3B (A3B) is normally an antiviral factor in the innate immune response. However, A3B has been implicated in cancer mutagenesis, particularly in solid tumors of the bladder, breast, cervix, head/neck, and lung. Here, we report data on the generation and characterization of a rabbit monoclonal antibody (mAb) for human A3B. One mAb, 5210-87-13, demonstrates utility in multiple applications, including ELISA, immunoblot, immunofluorescence microscopy, and immunohistochemistry. In head-to-head tests with commercial reagents, 5210-87-13 was the only rabbit monoclonal suitable for detecting native A3B and for immunohistochemical quantification of A3B in tumor tissues. This novel mAb has the potential to enable a wide range of fundamental and clinical studies on A3B in human biology and disease.

Keywords: APOBEC3B; DNA cytosine deaminase; IHC assay; cancer biomarker; rabbit monoclonal antibody.

Abstract

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