Next-generation sequencing allows the analysis of genomes, including those representing
disease states. However, the causes of most disorders are multifactorial, and systems-level …

Here we review recent developments and trends in sample preparation, pre-fractionation,
chromatography and mass spectrometry contributing towards the ultra-sensitive global …

Over the past decade peptide sequencing by collision induced dissociation (CID) has
become the method of choice in mass spectrometry-based proteomics. The development of …

Increasing peptide sequence coverage by tandem mass spectrometry improves confidence
in database search-based peptide identification and facilitates mapping of post-translational …

Surface metallization by plasma coating enhances desorption/ionization of membrane
components such as lipids and sterols in imaging time-of-flight secondary ion mass spectrometry (…

Microtubules are cytoskeletal polymers with two structurally and functionally distinct ends,
the plus- and the minus-end. Here, we focus on the mechanisms underlying the regulation of …

We recently introduced a novel scheme combining electron-transfer and higher-energy
collision dissociation (termed EThcD), for improved peptide ion fragmentation and identification. …

Axon formation, the initial step in establishing neuronal polarity, critically depends on local
microtubule reorganization and is characterized by the formation of parallel microtubule …

10.7554/eLife.07118.001 To maintain cellular structure and integrity during division, Gram-negative
bacteria must carefully coordinate constriction of a tripartite cell envelope of inner …

Although protein acetylation is widely observed, it has been associated with few specific
regulatory functions making it poorly understood. To interrogate its functionality, we analyzed …