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… at 95 C for 10 min, rapidly cooled to 0 C in an ice bath, and then incubated for another 10
min at … solution, we incubated the mixtures in the dark at room temperature for 2 h, after which …

… Critically, the use of heat-labile UDG enables the UDG-LAMP assay to be performed in a
single closed vessel, because the enzyme is rapidly and automatically deactivated when the …

… We then measured red reporter release at various concentrations of Hg 2+ to obtain the
IC 50 : the concentration at which the red fluorescence drops to half its maximum value. All …

… Testing this with a single intravenous injection (20 mg kg −1 ) of irinotecan we find that the
signal observed at both 10 and 120 Hz respond to the drug, but these are also accompanied …

… Second, all of our selection steps were done at 4 C at which the cells’ response to stimuli
is greatly decreased (38–40). However, we recognize other cell types (eg, endothelial cells) …

… by adding 50 μL of protein solution (at 25 nM unless noted otherwise) and incubating at 4
C for 37 h. All subsequent steps were performed at room temperature. After incubation, we …

… We opted for this approach because the alkyne side-chain at the 5-position of uracil
adopts an outward-pointing conformation in the major groove of the double helix, (26) which …

… 49 ), and the FSC collection angle is at most ±7 centred at 0 relative to the angle of incidence,
with a vertical bar blocking at least ±0.25 at the centre (based on the measured distances …

… at 100 C for 10 min, rapidly cooled in an ice bath for 10 min, and finally allowed to reach
room temperature. We incubated 2 nM thrombin at 10 C overnight with aptamers prepared at a …