… settling at intermediate phosphorylation states. This type of regulation is known to be critical
at different stages of mitosis. For example, robust mitotic entry is based on a bistable switch [ …

… effects of each enzyme into one overarching schematic of regulation at the Knl1/Mis12/Ndc80
(KMN) network (a major signaling hub at the outer kinetochore). This schematic will then …

… Our ability to detect some sulfenic acids at least may reflect the use of a high concentration
of maleimide, which rapidly alkylates low molecular weight and protein thiols that might …

… As sister kinetochores are bioriented, this results at equilibrium in a position at the equator
of the mitotic spindle. However, while research in the kinetochore field has made steady …

… both supernatants were then centrifuged at 16 800 g for 10 … 5) and ultracentrifuged at 100
000 g for 30 min at 4C. Pellets … -isolated by ultracentrifugation at 100 000 g for 30 min at 4C. …

… is shown that selective inhibition of PKCɛ halts cells at the final stages of separation. This
arrest is associated with persistent RhoA activation at the midbody and a delay in actomyosin …

… at the onset of mitosis. Live monitoring of cyclin B degradation reveals that this is essential to
establish the mitotic checkpoint quickly at … mitotic checkpoint rapidly at the onset of mitosis. …

… At 15 minutes but not 7 minutes of exposure, there was robust activation of p38-MAPK.
Figure 4A shows that the activation at 15 minutes is unaltered by coincident SB203580 at a …

… This demonstrates that staurosporine blocks Bim I–induced phosphorylation at all priming
… responsible for phosphorylation at any of the three sites (for example, PDK1 at the activation …

… antibodies for 2–4 h at room temperature or 16 h at 4 C, washed with PBS and incubated
with secondary antibodies for an additional hour at room temperature. Coverslips were then …