User profiles for A. T. Wassie
Asmamaw WassieMIT - Massachusetts Institute of Technology Verified email at mit.edu Cited by 2247 |
Expansion microscopy: principles and uses in biological research
Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial
resolution. We recently discovered that preserved biological specimens can be physically …
resolution. We recently discovered that preserved biological specimens can be physically …
Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems
… We localized genes that had been found in dendrites of CA1 pyramidal cells at the protein
level but had not been mapped at the mRNA level, such as γ-aminobutyric acid (GABA) type A …
level but had not been mapped at the mRNA level, such as γ-aminobutyric acid (GABA) type A …
Nanoscale imaging of RNA with expansion microscopy
… For DNAse I, samples were treated with DNAse I at 0.5 U/μL for 6 h at room temperature.
For formamide stripping, samples were treated with 100% formamide at 6 h at 37 C. …
For formamide stripping, samples were treated with 100% formamide at 6 h at 37 C. …
Iterative expansion microscopy
We recently developed a method called expansion microscopy, in which preserved
biological specimens are physically magnified by embedding them in a densely crosslinked …
biological specimens are physically magnified by embedding them in a densely crosslinked …
[PDF][PDF] Clonal decomposition and DNA replication states defined by scaled single-cell genome sequencing
…, D Goodwin, E Karagiannis, A Sinha, AT Wassie… - Cell, 2019 - cell.com
… First, we show that whole-chromosome aneuploidy, which occurs at low prevalence and
does not result in clonal amplification, is visible as whole-chromosome gains and losses at a …
does not result in clonal amplification, is visible as whole-chromosome gains and losses at a …
Expansion microscopy: protocols for imaging proteins and RNA in cells and tissues
… Incubate the cells with secondary antibodies in blocking buffer on a shaker at low speed
for 2 to 4 hr at room temperature or overnight at 4C. If using antibodies conjugated with dyes, …
for 2 to 4 hr at room temperature or overnight at 4C. If using antibodies conjugated with dyes, …
Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy
… Many crowded, biomolecule-rich structures are at the core of biological functions and …
decrowding effect of ExR, with both being imaged at the same level of resolution, we demonstrate …
decrowding effect of ExR, with both being imaged at the same level of resolution, we demonstrate …
Expansion microscopy of C. elegans
… at room temperature, and then for 4 hr at 4C (Figure 1B). We then incubate the animals in a
buffer containing 2% b-mercaptoethanol overnight at … 7.0 and 2 M NaCl) overnight at 4C. We …
buffer containing 2% b-mercaptoethanol overnight at … 7.0 and 2 M NaCl) overnight at 4C. We …
Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses
We present barcoded oligonucleotides ligated on RNA amplified for multiplexed and
parallel insitu analyses (BOLORAMIS), a reverse transcription-free method for spatially-resolved, …
parallel insitu analyses (BOLORAMIS), a reverse transcription-free method for spatially-resolved, …
[PDF][PDF] Spatial multiplexing of fluorescent reporters for imaging signaling network dynamics
… We found that, by fusing a fluorescent reporter to a pair of self-assembling peptides, it
could be stably clustered within cells at random points, distant enough to be resolved by a …
could be stably clustered within cells at random points, distant enough to be resolved by a …