Growing evidence shows that epigenetic mechanisms contribute to complex traits, with
implications across many fields of biology. In plant ecology, recent studies have attempted to …

Transposon-based gene vectors have become indispensable tools in vertebrate genetics
for applications ranging from insertional mutagenesis and transgenesis in model species to …

An ideal tool for gene therapy would enable efficient gene integration at predetermined
sites in the human genome. Here we demonstrate biased genome-wide integration of the …

The inherent risks associated with vector insertion in gene therapy need to be carefully
assessed. We analyzed the genome-wide distributions of Sleeping Beauty (SB) and piggyBac (…

Next generation sequencing is a common and versatile tool for biological and medical
research. We describe the basic steps for analyzing next generation sequencing data, including …

Emerging infectious diseases (EIDs) have contributed significantly to the current biodiversity
crisis, leading to widespread epidemics and population loss. Owing to genetic variation in …

Background Organisms typically face infection by diverse pathogens, and hosts are thought
to have developed specific responses to each type of pathogen they encounter. The advent …

Adeno-associated virus type 2 (AAV) is known to establish latency by preferential integration
in human chromosome 19q13.42. The AAV non-structural protein Rep appears to target a …

RBM10 encodes an RNA binding protein. Mutations in RBM10 are known to cause multiple
congenital anomaly syndrome in male humans, the TARP syndrome. However, the …

Helitron transposons capture and mobilize gene fragments in eukaryotes, but experimental
evidence for their transposition is lacking in the absence of an isolated active element. Here …