Protein splicing in trans by split inteins has increasingly become a powerful protein-engineering
tool for protein ligation, both in vivo and in vitro. Over 100 naturally occurring and …

Silk and cellulose are biopolymers that show strong potential as future sustainable materials.
They also have complementary properties, suitable for combination in composite materials …

A central concept in molecular bioscience is how structure formation at different length
scales is achieved. Here we use spider silk protein as a model to design new recombinant …

Segmental isotopic labeling is a powerful labeling technique for reducing nuclear magnetic
resonance (NMR) signal overlap, which is associated with larger proteins by incorporating …

Background Protein trans-splicing by naturally occurring split DnaE inteins is used for protein
ligation of foreign peptide fragments. In order to widen biotechnological applications of …

Naturally split DnaE intein from Nostoc punctiforme (Npu) has robust protein trans-splicing
activity and high tolerance of sequence variations at the splicing junctions. We determined the …

Phase transitions have an essential role in the assembly of nature’s protein-based materials
into hierarchically organized structures, yet many of the underlying mechanisms and …

Protein sequences are diversified on the DNA level by recombination and mutation and can
be further increased on the RNA level by alternative RNA splicing, involving introns that …

Protein splicing is an autocatalytic process involving self-excision of an internal protein
domain, the intein, and concomitant ligation of the two flanking sequences, the exteins, with a …

Many proteins, including cellular signaling proteins, cellsurface receptors, and modular
enzymes, are constructed from individual domains and connecting linkers.[1] Structural analysis …