Here we describe a protocol for advanced CUBIC (Clear, Unobstructed Brain/Body Imaging
Cocktails and Computational analysis). The CUBIC protocol enables simple and efficient …

Mammalian bodies have more than a billion cells per cubic centimeter, which makes whole-body
cell (WBC) profiling of an organism one of the ultimate challenges in biology and …

Whole-organ/body three-dimensional (3D) staining and imaging have been enduring
challenges in histology. By dissecting the complex physicochemical environment of the staining …

Spaceflight causes a decrease in skeletal muscle mass and strength. We set two murine
experimental groups in orbit for 35 days aboard the International Space Station, under artificial …

The MafB transcription factor is expressed in pancreatic α and β cells during development
but becomes exclusive to α cells in adult rodents. Mafb-null (Mafb −/− ) mice were reported to …

Myofibers are broadly characterized as fatigue-resistant slow-twitch (type I) fibers and rapidly
fatiguing fast-twitch (type IIa/IIx/IIb) fibers. However, the molecular regulation of myofiber …

Genome editing can introduce designed mutations into a target genomic site. Recent
research has revealed that it can also induce various unintended events such as structural …

During the maturation of transfer RNA (tRNA), a variety of chemical modifications can be
introduced at specific nucleotide positions post-transcriptionally. 5-Methyluridine (m 5 U) is one …

Focal segmental glomerulosclerosis (FSGS) is a common cause of steroid-resistant nephrotic
syndrome. Spontaneous remission of FSGS is rare and steroid-resistant FSGS frequently …

Genetically engineered mouse models are essential tools for understanding mammalian
gene functions and disease pathogenesis. Genome editing allows the generation of these …