User profiles for Akihito Fukudome

Akihito Fukudome

Howard Hughes Medical Institute / Department of Biology, Indiana University Bloomington
Verified email at iu.edu
Cited by 556

Plant dicer-like proteins: double-stranded RNA-cleaving enzymes for small RNA biogenesis

A Fukudome, T Fukuhara - Journal of Plant Research, 2017 - Springer
Dicer, a double-stranded RNA (dsRNA)-specific endoribonuclease, plays an essential role
in triggering both transcriptional and post-transcriptional gene silencing in eukaryotes by …

Distinct substrate specificities of Arabidopsis DCL3 and DCL4

H Nagano, A Fukudome, A Hiraguri… - Nucleic acids …, 2014 - academic.oup.com
In Arabidopsis thaliana, Dicer-like 3 (DCL3) and Dicer-like 4 (DCL4) cleave long, perfect
double-stranded RNAs (dsRNAs) into 24 and 21 nucleotides (nt) small interfering RNAs, …

Specific requirement of DRB4, a dsRNA-binding protein, for the in vitro dsRNA-cleaving activity of Arabidopsis Dicer-like 4

A Fukudome, A Kanaya, M Egami, Y Nakazawa… - Rna, 2011 - rnajournal.cshlp.org
Arabidopsis thaliana Dicer-like 4 (DCL4) produces 21-nt small interfering RNAs from both
endogenous and exogenous double-stranded RNAs (dsRNAs), and it interacts with DRB4, a …

[HTML][HTML] Double-stranded RNA sequencing reveals distinct riboviruses associated with thermoacidophilic bacteria from hot springs in Japan

S Urayama, A Fukudome, M Hirai, T Okumura… - Nature …, 2024 - nature.com
Metatranscriptome sequencing expanded the known diversity of the bacterial RNA virome,
suggesting that additional riboviruses infecting bacterial hosts remain to be discovered. Here …

Structure and RNA template requirements of Arabidopsis RNA-DEPENDENT RNA POLYMERASE 2

A Fukudome, J Singh, V Mishra… - Proceedings of the …, 2021 - National Acad Sciences
RNA-dependent RNA polymerases play essential roles in RNA-mediated gene silencing in
eukaryotes. In Arabidopsis, RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) physically …

Purification and characterization of Arabidopsis thaliana oligosaccharyltransferase complexes from the native host: a protein super‐expression system for structural …

…, S Lee, F Bonkhofer, J Tolley, A Fukudome… - The Plant …, 2018 - Wiley Online Library
The oligosaccharyltransferase ( OT ) complex catalyzes N‐glycosylation of nascent secretory
polypeptides in the lumen of the endoplasmic reticulum. Despite their importance, little is …

A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation

A Loffer, J Singh, A Fukudome, V Mishra, F Wang… - Elife, 2022 - elifesciences.org
In plants, selfish genetic elements, including retrotransposons and DNA viruses, are
transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short …

Assembly of a dsRNA synthesizing complex: RNA-DEPENDENT RNA POLYMERASE 2 contacts the largest subunit of NUCLEAR RNA POLYMERASE IV

…, F Wang, Y Zhang, A Fukudome… - Proceedings of the …, 2021 - National Acad Sciences
In plants, transcription of selfish genetic elements such as transposons and DNA viruses is
suppressed by RNA-directed DNA methylation. This process is guided by 24-nt short-…

[HTML][HTML] Regulation of abiotic stress signalling by Arabidopsis C-terminal domain phosphatase-like 1 requires interaction with a k-homology domain-containing protein

IS Jeong, A Fukudome, E Aksoy, WY Bang, S Kim… - PLoS …, 2013 - journals.plos.org
Arabidopsis thaliana CARBOXYL-TERMINAL DOMAIN (CTD) PHOSPHATASE-LIKE 1 (CPL1)
regulates plant transcriptional responses to diverse stress signals. Unlike typical CTD …

Arabidopsis CPL4 is an essential C‐terminal domain phosphatase that suppresses xenobiotic stress responses

A Fukudome, E Aksoy, X Wu, K Kumar… - The Plant …, 2014 - Wiley Online Library
Eukaryotic gene expression is both promoted and inhibited by the reversible phosphorylation
of the C‐terminal domain of RNA polymerase II (pol II CTD). More than 20 Arabidopsis …