Antimicrobial peptides are promising alternative antimicrobial agents. However, little is
known about whether resistance to small-molecule antibiotics leads to cross-resistance (…

Currently available tools for multiplex bacterial genome engineering are optimized for a few
laboratory model strains, demand extensive prior modification of the host strain, and lead to …

Metabolic engineering holds the promise to transform the chemical industry and to support
the transition into a circular bioeconomy, by engineering cellular biocatalysts that efficiently …

Understanding how evolution of antimicrobial resistance increases resistance to other drugs
is a challenge of profound importance. By combining experimental evolution and genome …

Antibiotic development is frequently plagued by the rapid emergence of drug resistance.
However, assessing the risk of resistance development in the preclinical stage is difficult. …

Engineering the genetic code of an organism has been proposed to provide a firewall from
natural ecosystems by preventing viral infections and gene transfer 1 , 2 , 3 , 4 , 5 – 6 . …

The human gut microbiota has adapted to the presence of antimicrobial peptides (AMPs),
which are ancient components of immune defence. Despite its medical importance, it has …

Evidence suggests that novel enzyme functions evolved from low‐level promiscuous activities
in ancestral enzymes. Yet, the evolutionary dynamics and physiological mechanisms of …

Evolution of antibiotic resistance in microbes is frequently achieved by acquisition of
spontaneous mutations during antimicrobial therapy. Here, we demonstrate that inactivation of a …

The ideal bacterial chassis provides a simplified, stable and predictable host environment
for synthetic biological circuits. Mutability and evolution can, however, compromise stability, …