Single-point fluorescence correlation spectroscopy (FCS) allows measurements of fast
diffusion and dynamic processes in the microsecond-to-millisecond time range. For …

Images obtained with a laser-scanning microscope contain a time structure that can be
exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation …

Understanding how a subset of expressed genes dictates cellular phenotype is a considerable
challenge owing to the large numbers of molecules involved, their combinatorics and the …

We describe a method to detect molecular complexes and measure their stoichiometry in
living cells from simultaneous fluctuations of the fluorescence intensity in two image channels, …

Background A complex network of putative molecular interactions underlies the architecture
and function of cell-matrix adhesions. Most of these interactions are implicated from …

Paxillin is an adaptor molecule involved in the assembly of focal adhesions. Using different
fluorescence fluctuation approaches, we established that paxillin-EGFP is dynamic on many …

We describe a general method for detecting molecular complexes based on the analysis of
single molecule fluorescence fluctuations from laser scanning confocal images. The method …

Talin and vinculin are mechanosensitive proteins that are recruited early to integrin-based
nascent adhesions (NAs). In two epithelial cell systems with well-delineated NA formation, we …

Brain injuries such as trauma and stroke lead to glial scar formation by reactive astrocytes
which produce and secret axonal outgrowth inhibitors. Chondroitin sulfate proteoglycans (…

The connective tissue formed by extracellular matrix (ECM) rich in fibronectin and collagen
consists a barrier that cancer cells have to overpass to reach blood vessels and then a …