Towards community-driven metadata standards for light microscopy: tiered specifications extending the OME model

M Hammer, M Huisman, A Rigano, U Boehm… - Nature …, 2021 - nature.com
Rigorous record-keeping and quality control are required to ensure the quality, reproducibility
and value of imaging data. The 4DN Initiative and BINA here propose light Microscopy …

[HTML][HTML] Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications

A Rigano, S Ehmsen, SU Öztürk, J Ryan, A Balashov… - Nature …, 2021 - nature.com
For quality, interpretation, reproducibility and sharing value, microscopy images should be
accompanied by detailed descriptions of the conditions that were used to produce them. Micro…

OMEGA: a software tool for the management, analysis, and dissemination of intracellular trafficking data that incorporates motion type classification and quality control

A Rigano, V Galli, JM Clark, LE Pereira, L Grossi… - BioRxiv, 2018 - biorxiv.org
MOTIVATION Particle tracking coupled with time-lapse microscopy is critical for understanding
the dynamics of intracellular processes of clinical importance. Spurred on by advances in …

Proposal for minimum information guidelines to report and reproduce results of particle tracking and motion analysis

A Rigano, C Strambio-De-Castillia - bioRxiv, 2017 - biorxiv.org
The proposed Minimum Information About Particle Tracking Experiments (MIAPTE) reporting
guidelines described here aim to deliver a set of rules representing the minimal information …

[PDF][PDF] Microscopy metadata: more important and less boring than you think

M Hammer, A Rigano, C Strambio-De-Castillia… - Biophysical …, 2022 - cell.com
The intense use of microscopes in the biomedical and basic sciences, combined with ever
better and more versatile new microscopy methods becoming commercially available, has …

[PDF][PDF] The 4DN-OME Ontology: an OME-OWL Extension with Emphasis on Usability, Minimum Information Guidelines and Quality Control for Super-resolution …

M Hammer, A Rigano, F Farzam, M Huisman… - SWAT4HCLS, 2019 - ceur-ws.org
The Open Microscopy Environment (OME) model [1] is a specification for sharing biological
imaging data that stores metadata as OME-XML. The OME Consortium recently introduced …

An algorithm-centric Monte Carlo method to empirically quantify motion type estimation uncertainty in single-particle tracking

A Rigano, V Galli, K Gonciarz, IF Sbalzarini… - bioRxiv, 2018 - biorxiv.org
Quantitative analysis of microscopy images is ideally suited for understanding the functional
biological correlates of individual molecular species identified by one of the several …

Author Correction: Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications

A Rigano, S Ehmsen, SU Öztürk, J Ryan… - Nature …, 2022 - ncbi.nlm.nih.gov
In the version of this article initially published, the affiliation for author Roland Nitschke was
incorrectly formulated. The affiliation has been corrected to read “Life Imaging Center and …

[HTML][HTML] MethodsJ2

J Ryan, T Pengo, A Rigano, PM Llopis, MS Itano… - 2022 - protocols.io
DISCLAIMER: As per MethodsJ, this is not meant to be applied blindly, but rather to be used
as a starting point. Metadata is recorded by the microscope driving software, so at best it re …