Here we describe a method for fabricating a primary human Small Intestine-on-a-Chip (Intestine
Chip) containing epithelial cells isolated from healthy regions of intestinal biopsies. The …

The diverse bacterial populations that comprise the commensal microbiome of the human
intestine play a central role in health and disease. A method that sustains complex microbial …

… Author links open overlay panel Amir Bein 1 a , Woojung Shin 2 a , Sasan Jalili-Firoozinezhad
1 3 , Min Hee Park 2 , Alexandra Sontheimer-Phelps 1 4 , Alessio Tovaglieri 1 5 , Angeliki …

Clostridium difficile toxin B (TcdB) is a critical virulence factor that causes diseases
associated with C. difficile infection. Here we carried out CRISPR–Cas9-mediated genome-wide …

Background & Aims The mucus layer in the human colon protects against commensal
bacteria and pathogens, and defects in its unique bilayered structure contribute to intestinal …

The gastrointestinal (GI) epithelium is a highly regenerative tissue with the potential to provide
a renewable source of insulin + cells after undergoing cellular reprogramming. Here, we …

Background Species-specific differences in tolerance to infection are exemplified by the high
susceptibility of humans to enterohemorrhagic Escherichia coli (EHEC) infection, whereas …

The cellular and molecular mechanisms underlying adaptive changes to physiological
stress within the intestinal epithelium remain poorly understood. Here, we show that PTEN, a …

The intestinal epithelium serves as an essential barrier to the outside world and is maintained
by functionally distinct populations of rapidly cycling intestinal stem cells (CBC ISCs) and …

Inflammatory bowel disease (IBD) is a chronic condition driven by loss of homeostasis between
the mucosal immune system, the commensal gut microbiota, and the intestinal epithelium…