Thiamin and riboflavin are precursors of essential coenzymes—thiamin pyrophosphate (TPP)
and flavin mononucleotide (FMN)/flavin adenine dinucleotide (FAD), respectively. In …

Aptamers are artificial nucleic acids that selectively bind small molecules. In the past two years,
it has become clear that nature has already devised its own aptamers that play important …

Newly synthesized procollagen type I (PC) assembles into 300 nm rigid, rod-like triple helices
in the lumen of the endoplasmic reticulum. This oligomeric complex moves to the Golgi …

Transport intermediates (TIs) have a central role in intracellular traffic, and much effort has
been directed towards defining their molecular organization. Unfortunately, major …

The organization of secretory traffic remains unclear, mainly because of the complex structure
and dynamics of the secretory pathway. We have thus studied a simplified system, a single …

Many operons in Gram-positive bacteria that are involved in methionine (Met) and cysteine (Cys)
biosynthesis possess an evolutionarily conserved regulatory leader sequence (S-box) …

Protein transport between the ER and the Golgi in mammalian cells occurs via large pleiomorphic
carriers, and most current models suggest that these are formed by the fusion of small …

As with other complex cellular functions, intracellular membrane transport involves the
coordinated engagement of a series of organelles and machineries; however, the molecular basis …

Procollagen (PC)-I aggregates transit through the Golgi complex without leaving the lumen
of Golgi cisternae. Based on this evidence, we have proposed that PC-I is transported across …

Newly synthesized proteins exit the endoplasmic reticulum (ER) via coat protein complex II (COPII)
vesicles. Procollagen (PC), however, forms prefibrils that are too large to fit into …