Exploring the ability of organisms to locally adapt is critical for determining the outcome of
rapid climate changes, yet few studies have addressed this question in microorganisms. We …

We examined the northern limit of Saccharomyces cerevisiae and Saccharomyces
paradoxus in northeast America. We collected 876 natural samples at 29 sites and applied …

Protein–protein interactions (PPIs) between modular binding domains and their target peptide
motifs are thought to largely depend on the intrinsic binding specificities of the domains. …

Base editors derived from CRISPR-Cas9 systems and DNA editing enzymes offer an
unprecedented opportunity for the precise modification of genes, but have yet to be used at a …

Since deleterious mutations may be rescued by secondary mutations during evolution,
compensatory evolution could identify genetic solutions leading to therapeutic targets. Here, we …

Cellular processes and homeostasis control in eukaryotic cells is achieved by the action of
regulatory proteins such as protein kinase A (PKA). Although the outbound signals from PKA …

Gene duplication is ubiquitous and a major driver of phenotypic diversity across the tree of
life, but its immediate consequences are not fully understood. Deleterious effects would …

The evolution of protein-coding genes proceeds as mutations act on two main dimensions:
regulation of transcription level and the coding sequence. The extent and impact of the …

Little is known about the rate of emergence of de novo genes, what their initial properties are,
and how they spread in populations. We examined wild yeast populations (…

10.7554/eLife.46754.001 Gene duplication is a driver of the evolution of new functions. The
duplication of genes encoding homomeric proteins leads to the formation of homomers and …