We have determined the complete 1,694,969-nt sequence of the GC-rich genome of
Methanopyrus kandleri by using a whole direct genome sequencing approach. This approach is …

Thermostable DNA polymerases have a crucial role in current methods of DNA amplification
and sequencing. Major improvements in these methods have been made in recent years, …

Helix–hairpin–helix (HhH) is a widespread motif involved in sequence-nonspecific DNA
binding. The majority of HhH motifs function as DNA-binding modules with typical occurrence of …

The genomes of two closely related lytic Thermus thermophilus siphoviruses with exceptionally
long (∼800 nm) tails, bacteriophages P23-45 and P74-26, were sequenced completely. …

Topoisomerase V is a type I topoisomerase without structural or sequence similarities to
other topoisomerases. Although it belongs to the type I subfamily of topoisomerases, it is …

Topoisomerases are involved in controlling and maintaining the topology of DNA and are
present in all kingdoms of life. Unlike all other types of topoisomerases, similar type IB …

Enzymes from (hyper)thermophiles “Thermozymes” offer a great potential for biotechnological
applications. Thermophilic adaptation does not only provide stability toward high …

The robust detection of structural variants in mammalian genomes remains a challenge. It is
particularly difficult in the case of genetically unstable Chinese hamster ovary (CHO) cell …

Previously we have characterized type IB DNA topoisomerase V (topo V) in the hyperthermophile
Methanopyrus kandleri. The enzyme has a powerful topoisomerase activity and is …

Archaeal histones have significant sequence and structural similarity to their eukaryal
counterparts. However, whereas DNA is wrapped in negatively constrained supercoils in eukaryal …