Although metabolism plays an active role in antibiotic lethality, antibiotic resistance is generally
associated with drug target modification, enzymatic inactivation, and/or transport rather …

Antibiotics target energy-consuming processes. As such, perturbations to bacterial metabolic
homeostasis are significant consequences of treatment. Here, we describe three postulates …

It is generally assumed that antibiotics can promote horizontal gene transfer. However, because
of a variety of confounding factors that complicate the interpretation of previous studies, …

Growth rate and metabolic state of bacteria have been separately shown to affect antibiotic
efficacy 1 , 2 – 3 . However, the two are interrelated as bacterial growth inherently imposes a …

In the absence of antibiotic-mediated selection, sensitive bacteria are expected to displace
their resistant counterparts if resistance genes are costly. However, many resistance genes …

Current machine learning techniques enable robust association of biological signals with
measured phenotypes, but these approaches are incapable of identifying causal relationships. …

Predictive biology is the next great chapter in synthetic and systems biology, particularly for
microorganisms. Tasks that once seemed infeasible are increasingly being realized such as …

Bacteria have developed resistance against every antibiotic at a rate that is alarming considering
the timescale at which new antibiotics are developed. Thus, there is a critical need to …

Conjugative plasmids drive genetic diversity and evolution in microbial populations. Despite
their prevalence, plasmids can impose long-term fitness costs on their hosts, altering …

… Allison J Lopatkin Allison J Lopatkin … We would also like to thank J. Yang for helpful discussions
on data interpretation. This study was funded by the National Science Foundation award …