It has long been the dream of biologists to map gene expression at the single-cell level.
With such data one might track heterogeneous cell sub-populations, and infer regulatory …

Intestinal stem cells, characterized by high Lgr5 expression, reside between Paneth cells at
the small intestinal crypt base and divide every day. We have carried out fate mapping of …

Although the function of the mammalian pancreas hinges on complex interactions of distinct
cell types, gene expression profiles have primarily been described with bulk mixtures. Here …

… ,3 and Allon M. Klein1… from the original gene expression space to a new space U that is
RM (M<N), or in the case of additional discrete cluster assignment, U is Z3RM (possibly with …

… Further information and requests for resources and reagents should be directed to and will
be fulfilled by the Lead Contact, Allon M. Klein (allon_klein@hms.harvard.edu). Raw RNA …

According to the current model of adult epidermal homeostasis, skin tissue is maintained by
two discrete populations of progenitor cells: self-renewing stem cells; and their progeny, …

The functions of epithelial tissues are dictated by the types, abundance and distribution of
the differentiated cells they contain. Attempts to restore tissue function after damage require …

High-throughput mapping of cellular differentiation hierarchies from single-cell data promises
to empower systematic interrogations of vertebrate development and disease. Here we …

Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular
heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for …

With the capacity for rapid self-renewal and regeneration, the intestinal epithelium is stereotypical
of stem cell–supported tissues. Yet the pattern of stem cell turnover remains in question…