Cas9 targets genomic loci with high specificity. For knockin with double-strand break repair,
however, Cas9 often leads to unintended on-target knockout rather than intended edits. This …

The kinase mTOR is a signaling hub for pathways that regulate cellular growth. In neurons,
the subcellular localization of mTOR takes on increased significance. Here, we review …

Mutations in nitrogen permease regulator-like 3 (NPRL3), a component of the GATOR1
complex within the mTOR pathway, are associated with epilepsy and malformations of cortical …

Background Neuroligin-3 is a postsynaptic adhesion molecule involved in synapse development
and function. It is implicated in rare, monogenic forms of autism, and its shedding is …

Neuroligin-3 is a postsynaptic adhesion molecule involved in development, function, and
pathologies of synapses in the brain. It is a genetic cause of autism and a potent component of …

Current Cas9 reagents can target genomic loci with high specificity. However, when used for
knockin, on-target outcomes are inherently imprecise, often leading to unintended knockout …

Generating animal models for individual patients within clinically relevant time frames holds
the potential to revolutionize personalized medicine for rare genetic epilepsies. By …

Specific and highly diverse connectivity between functionally specialized regions of the
nervous system is controlled at multiple scales, from anatomically organized connectivity …

Human milk pepsin and pancreatin digests were separated using molecular membrane and
reverse phase chromatography. Chemical screening of the resulting fractions using the …

Signal transduction is used by plants to coordinate their development and to sense and
respond to fluctuations in their surroundings. With previous proteomics approaches, we …