Essential genes encode the processes that are necessary for life. Until recently, commonly
applied binary classifications left no space between essential and non-essential genes. In …

Genetic tools are critical to dissecting the mechanisms governing cellular processes, from
fundamental physiology to pathogenesis. Members of the genus Burkholderia have potential …

Novel therapies are urgently needed to alleviate the current crisis of multiple drug-resistant
infections. The bacterial signal transduction mechanisms, known as two-component systems (…

Motivation Chemical–genetic interaction profiling is a genetic approach that quantifies the
susceptibility of a set of mutants depleted in specific gene product(s) to a set of chemical …

The cell envelope of Gram-negative bacteria belonging to the Burkholderia cepacia complex
(Bcc) presents unique restrictions to antibiotic penetration. As a consequence, Bcc species …

To streamline the elucidation of antibacterial compounds’ mechanism of action, comprehensive
high-throughput assays interrogating multiple putative targets are necessary. However, …

The glycophosphatidylinositol‐anchored cell surface receptor CD 24 (also called heat‐stable
antigen) promotes the apoptosis of progenitor and precursor B‐lymphocytes. However, the …

The rise in antibiotic resistance among pathogenic microorganisms has created an imbalance
in the drugs available for treatment, in part due to the slow development of new antibiotics. …

A diverse genetic toolkit is critical for understanding bacterial physiology and genotype-phenotype
relationships. Inducible promoter systems are an integral part of this toolkit. In …

Screening for novel antibacterial compounds in small molecule libraries has a low success
rate. We applied machine learning (ML)-based virtual screening for antibacterial activity and …