Plasma of cancer patients contains cell-free tumor DNA that carries information on tumor
mutations and tumor burden. Individual mutations have been probed using allele-specific …

High-throughput single-cell transcriptomics offers an unbiased approach for understanding
the extent, basis and function of gene expression variation between seemingly identical cells…

Large-scale surveys of single-cell gene expression have the potential to reveal rare cell
populations and lineage relationships but require efficient methods for cell capture and mRNA …

We have created a compendium of single cell transcriptomic data from the model organism
Mus musculus comprising more than 100,000 cells from 20 organs and tissues. These data …

Decades of work have aimed to genetically reprogram T cells for therapeutic purposes 1 , 2
using recombinant viral vectors, which do not target transgenes to specific genomic sites 3 , …

The repair outcomes at site-specific DNA double-strand breaks (DSBs) generated by the
RNA-guided DNA endonuclease Cas9 determine how gene function is altered. Despite the …

RNA-guided CRISPR–Cas9 endonucleases are widely used for genome engineering, but
our understanding of Cas9 specificity remains incomplete. Here, we developed a biochemical …

The RNA-guided Cas9 endonuclease specifically targets and cleaves DNA in a sequence-dependent
manner and has been widely used for programmable genome editing. Cas9 …

Cancer genome sequencing studies have identified numerous driver genes, but the relative
timing of mutations in carcinogenesis remains unclear. The gradual progression from …

… The high failure rate of ETV in these infants may be explained by poor CSF absorption at
the arachnoid villi, resulting in hydrocephalus despite adequate communication between the …