The CRISPR–Cas9 system provides a versatile toolkit for genome engineering that can
introduce various DNA lesions at specific genomic locations. However, a better understanding of …

Defective DNA repair by homologous recombination (HR) is thought to be a major contributor
to tumorigenesis in individuals carrying Brca1 mutations. Here, we show that DNA breaks …

Chromosomal translocation requires formation of paired double-strand DNA breaks (DSBs)
on heterologous chromosomes. One of the most well characterized oncogenic translocations …

Class switch recombination (CSR) diversifies antibodies by joining highly repetitive DNA
elements, which are separated by 60–200 kbp. CSR is initiated by activation-induced cytidine …

53BP1 is a DNA damage protein that forms phosphorylated H2AX (γ-H2AX) dependent foci
in a 1 Mb region surrounding DNA double-strand breaks (DSBs). In addition, 53BP1 …

Chromosomal rearrangements, including translocations, require formation and joining of
DNA double strand breaks (DSBs). These events disrupt the integrity of the genome and are …

Tumor suppressor p53-binding protein 1 (53BP1) regulates the repair of dysfunctional telomeres
lacking the shelterin protein TRF2 by promoting their mobility, their nonhomologous end…

Research over the past decade has suggested important roles for pseudogenes in physiology
and disease. In vitro experiments demonstrated that pseudogenes contribute to cell …

Cancer-initiating translocations such as those associated with lymphomas require the formation
of paired DNA double-strand breaks (DSBs). Activation-induced cytidine deaminase (AID…

Cells repair DNA double-strand breaks (DSBs) through a complex set of pathways critical for
maintaining genomic integrity. To systematically map these pathways, we developed a high-…